Identification of Metastatic Biomarkers for Head and Neck Squamous Cell Carcinoma by Liquid Chromatography-Tandem Mass Spectrometry and Array-Based Comparative Genomic Hybridization - ICMS2015 Abstract Submission Form Tumor Microenvironment Conference

author.DisplayName ¹ author.DisplayName ² author.DisplayName ³ author.DisplayName ⁴ author.DisplayName ⁴ author.DisplayName ¹

¹Oral Biology, Faculty of Dentistry, Thammasat University, Thailand
²Proteomics Research Laboratory, National Center for Genetic Engineering and Biotechnology, Thailand
³Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Thailand
⁴Genetics, Laboratory of Animal Cytogenetics and Comparative Genomics, Faculty of Science, Kasetsart University, Thailand

Head and neck squamous cell carcinoma (HNSCC) is one of the most common types of human cancer with frequent recurrence and lymph node metastasis. Extremely poor prognosis in patients with locally advanced disease has been a major problem for decades. Therefore, it is urgent to discover metastasis-related biomarkers for the detection of HNSCC in its metastasis stage. Three pairs of HNSCC cell lines were used. HN18, HN30. HN4 were derived from primary lesions whereas HN17. HN31, HN12 were derived from lymph nodes metastasis lesions which belong to the same patients, respectively. Gel electrophoresis was performed using total cell lysate from paired primary and metastatic HNSCC cell lines. Liquid Chromatography-Tandem Mass Spectrometry was used for proteome analysis. Ribosomal protein S6 kinase 1 (RSK1) was identified as a candidate protein whose expression was associated with the metastatic stage of the HNSCC cell lines. Array-based comparative genomic hybridization (array CGH) was also performed in paired HNSCC cell lines to identify regions where deletion and duplication occurred across all chromosomes in paired HNSCC cell lines. Analysis of array CGH data revealed four candidate genes including SIX homeobox 3 (SIX3), H19, protein tyrosine phosphatase, receptor type, D (PTPRD) and WW domain containing oxidoreductase (WWOX) located on fragments of chromosomes where genomic alteration occurred in metastatic HNSCC cell lines. We validated the expression of the candidate biomarkers using realtime-PCR and Western blotting. The expression of RSK1, SIX3 and H19 was increased whereas lower expression of PTPRD was detected in metastatic HNSCC cell lines compared to its primary counterpart. However, the expression of WWOX was not detected. Together, we identified four candidate biomarkers whose expression was associated with metastatic HNSCC cell lines.