Research of Cytokine Expression Pattern when Endometrial Cancer Co Culture with Stroma

Recently tumor micro-environment study has research very much. cancer usually origin epithelial cells. so cancer surrounded by stroma cell, immune cells. And cancer give and take signals such as growth factor, chemokine, cytokines. These action called ‘cross-talk’. cancer cross talk with stroma, normal epithelial cell, and even immune cell. It provide cancer has more malignancy. so we did cytokine assay via endometrial cancer line co culture with endometrial stroma cells. and we perform Reverse transcription PCR. our result show that cytokine from co-culture with endometrial cancer and endometrial stroma release more than culture alone. and another pattern of expression observed in co-culture medium.