ELECTRIC FIELD ASSISTED ANTIBODY ORIENTATION

Over the years, contamination of naturally occurring resources like air and water is leading to an imbalance in the ecosystem of mankind. To prevent the harmful effects an early detection of the hazardous elements is inevitable. The diagnostic methods that are in practice until today have not met the demands of on-spot detection [1]. And to serve such applications, a device like biosensor can be used. A biosensor is composed of a sensing surface, transduction elements and detector to notify the changes that occur at the surface [2]. Antibodies (Ab) can be used for detection as they are selective and specific in binding to their complementary molecules which results in quantifiable biochemical interaction [3]. By structure Ab are asymmetric and thus they tend to orient randomly when immobilized [3]. And to immobilize Ab, here we’ve exploited its dipolar nature using electric field for regulating their behavior [4]^. The results of this procedure also include comparison of immobilization using: a) the presence of current inside the flowcell in PBS and b) to verify the influence of ions in the flow channel.

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