Modified Starch Based Nanoparticles as PIP₃ Delivery System for Wound Healing

Wound healing is a natural restorative response to tissue injury. The primary function of the skin is to serve as a protective barrier against the environment. Once the protective barrier is broken, as a result of an injury, the normal process of wound healing is immediately set in motion.Phosphatidylinositol-3,4,5-trisphosphate (PIP₃), is a phosphorylated phosphatidylinositol with phosphate moiety on positions 3,4 and 5 of the inositol ring. PIP₃ is a negatively charged required intracellular lipid second messenger, which plays an important role in the regulation of many cellular processes, including proliferation, growth, survival, migration and wound healing. One of the main challenges is creating an effective path for delivering negatively charged PIP₃ into keratinocytes cells. In this study, we use modified cationic starch (Q-Starch), which is a natural polysaccharide, as a putative PIP₃ nanocarrier. The positively charged Q-starch and the negatively charged PIP₃ interact electrostatically, to form a complex. The PIP₃/Q-Starch delivery system was developed and characterized, complexes were successfully obtained above 1.5 N/P ratio. The cellular delivery path was investigated.