Photonic Biomedical Mapping via Manipulated Magnetic Nano Particles

Abstract-

Our main goal is to develop a new speckle based imaging modality for biological applications. The method is based on detection and manipulation of targeted conjugated magnetic nanoparticles (MNPs), which can specifically target cells or other live tissue, and form a concentrated assembly yielding speckle imaging capabilities. The MNPs

detection technique employs temporal tracking of secondary speckle patterns while applying a magnetic excitation field that oscillates the particles.

It was found, that the resultant modulation spectrum of the speckle patterns is directly associated with the properties of the MNPs. In an ex vivo experiment we demonstrates the potential of the proposed method for detecting the presence of arterial plaque.

By taking those results into consideration, the method holds a great promise for non-invasive speckle imaging.

How it works-

• Coat the MNPs with different coatings types.
• Inject the particles to the blood stream.
• Apply an external magnetic excitation field.
• Illuminate the particles with laser.
• The scattered light is recorded by a camera.
• The video sequence is analyzed.

Speckle-

We use laser illumination that interacts with the MNPs and changes the typical speckle fingerprint of targeted tissue.

Detection-

Our aim is to have frequencies which differ from each other in order to allow us to differentiate between the different types of the particles used as biological markers.

Results-

The results demonstrate the ability of the algorithm to estimate the horizontal and vertical displacement of the particles under external magnetic field. The temporal information (flickering ) was Fourier transformed and the temporal frequency of the spectrum was analyzed as well as the amplitude.

Conclusion-

In this work we presented a new approach which facilitates speckle imaging by targeted MNPs of various dimensions by applying an external magnetic AC field has been demonstrated.

The proposed MNP based concept can potentially be developed for dual functionality; not only for detection, but for therapeutic purposes as well, yielding significant clinical applicability.