The processing of all types of hard material in the routine paraffin process in histology needs an additional decalcification step.
Routine hard material as bone, teeth or nails will be decalcified with inorganic acids normally.
For sensitive hard material e.g. bone marrow biopsies used with an acid decalcification solution should not be used because the nuclear structure, nucleic acids and especially anti-gene structures will be influenced negatively or might be inactivated completely.
Here will be presented how the results for sensitive and routine hard material can be enhanced by selection of the suitable decalcification agent and to use the right decalcification solution produce best result for immune histochemical methods and PCR which are based on the full anti-gene activity.
