· the substrate should contain open pores that connect to each other about the right size to inclusion of cells, then tissues and their vascularity
· they should have appropriate chemical properties (bioactivity, non-toxic) to promote attachment of cells to substrates, their differentiation and multiplication, mechanical properties (tensile strength, torsion, hardness, Young's modulus) close to the natural materials
· they should be made of materials with controlled biodegradability (biosorption),
so that tissue could be replaced after a specified time basis
· not cause adverse reactions (including allergic)
· they should be easily manufactured in various shapes and sizes.
Our scaffolds consists of submicrocrystalline sintered corundu as a matrix phase
and glass from CaO – SiO2 - P2O5 – Na2O system as a binder phase. The research program contains:
- determination of the raw materials mechanochemical treatment effect on their microstructure and physical – chemical properties (XRD, FTiR, DTA/DTG, grains fraction analysis – population curve, comulative curve SBET, HV, SEM observation);
- investigation of physical – chemical properties of biocomposite (real, helium, apparent density, SBET, porosity, SEM observation, calculation of thermodynamic stability
by VCS algorythm) with surface topography analysis (roughness profile material rate, amplitude distribution of the ordinates);
- research of chemical compound effect and the manner of manufacturing of scaffolds on the growth character of human skin fibroblast cells of CCL 110 line;
- work out a procedure of scaffolds manufacturing and their properties verification.
References
[1] Shi D.: Biomaterials and Tissue Engineering. Springer – Verlag, Berlin 2004
[2] Brovarone C.,Verne E.: Macroporous bioactive glass – ceramic scaffolds for tissue engineering. Journal of Materials Science: Materials in Medicine, Springer Science + Business Media, New York 2006
[3] Sachlos E.,Czernuszka J.T.: Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. European Cells and Materials, AO Research Institute Davos, Davos 2003
[4] Szarska S., Staniewicz – Brudnik B., Lekka M.: The effect of the size of the substrate grain made of submicrocrystalline sintered corundum on the bioglass composite structure and certain physico-mechanical properties of the bioglass. Optica Applicata, Institute of Physics, Wrocław University of Technology, Wrocław 2008
[5] Puttini S., Lekka M., Saugy D., Dorchies O., Incitti T., Bozzoni I., Kulik A.J., Mermod N.: Atomic force microscopy assay of the elasticity of utrophin-expressing muscles. Molecular Therapy, Molecular Therapy Editorial Office, Seattle 2009