Genetic Polymorphisms of Folate Pathway Enzymes in Women with Reproductive Failure - WCRPL2017 – 2nd World Congress on Recurrent Pregnancy Loss, 19-22, January 2017, Cannes, France

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¹laboratory of immunohematology, Kirov Research Institute of Hematology and Blood Transfusion, Russia
²research-clinical department, Kirov Research Institute of Hematology and Blood Transfusion, Russia
³doctor of hematology department, Kirov Research Institute of Hematology and Blood Transfusion, Russia
⁴gynecology department, Kirov Regional State Healthcare Agency "Yuryansky District Hospital", Russia
⁵director, Kirov Research Institute of Hematology and Blood Transfusion, Russia

Background: Currently there is no single view on the causes of reproductive disorders.Hyperhomocysteinemia and abnormal functioning of the processes of DNA methylation in somatic and germ cells, leading to loss of pregnancy or her non-coming are celebrated among the variety of mechanisms of fertility violation.Objective. To assess the frequency of genetic polymorphisms in folate cycle metabolic pathway (MTHFR: 677, 1298; MTR: 2756; MTRR: 66)in women with reproductive failures and without fertility disorders.

Methods: The study was carried out by PCR. Statistical data processing was performed using the χ2 criterion.

Genetic polymorphisms of folate pathway enzymes in 277 women were analyzed:

1 group – 30 women with primary infertility;

2 group – 84 who had in a anamnesis of preterm delivery and reproductive failures;
3 group – 32 with abnormal labors in obstetric history (preterm delivery, preeclampsia, abruption of placenta);

4 group – 37 with two missed abortions in the anamnesis;
5 group - 22 with three or more recurrent spontaneous abortions.
Control group - 72 women without obstetric history of 2 or more deliveries at term.

Results: The distribution pattern of homo- and heterozygous forms of genes involved in folate metabolism are presented in table.

Tabl. The distribution of occurrence of folate cycle enzymes in women with different pregnancy outcomes

The distribution of occurence of folate cycle enzymes in women with different pregnancy outcomes

Statistical analysis revealed no differences in the frequency of occurrence of allelic genes variants MTHFR: 677, MTHFR: 1298, MTR: 2756, MTRR: 66 in the group of women without reproductive failure, and in groups of patients with infertility, abnormal labor and missed abortions in the anamnesis (p>0.05).

Conclusions: In this study, the influence of gene polymorphisms of MTHFR: 677, 1298, MTR: 2756, MTRR: 66 on advent, course and outcome of pregnancy in women is not established.