Keynote Lecture
Chemiluminescence Probes with Color Modulation

Chemiluminescence is among the most sensitive methods for achieving a high signal-to-noise ratio in various chemical and biological applications. We have developed a modular practical synthetic route for preparation of turn-ON dioxetane-tethered fluorophore chemiluminescent probes. The chemiluminescent emission of the probes was significantly amplified through energy transfer mechanism under physiological conditions. Two probes were composed of dioxetane, tethered with green and near-infrared (NIR) fluorescent dyes. While both probes were able to provide chemiluminescence in vivo images following subcutaneous injection, only the NIR probe could provide chemiluminescence image following intraperitoneal injection. These are the first in vivo images produced by dioxetane-chemiluminescence probes with no addition of surfactant enhancer. We anticipate that our practical synthetic methodology will be useful for preparation of various chemiluminescent probes for numerous applications.