Clinical and Genetic Factors Associated with CAD in PAD Patients

Aim: Analysis of the relationship between clinical parameters, T-786C polymorphism of eNOs gene and presence of CAD in patients with PAD.
Materials and Methods: We examined 100 patients with PAD (lower limbs ischemia II–IV stages). 37,0 % of patients had concomitant CAD (group I), 63,0 % of patients did not have CAD (group II). The patients’ survey included the coronary angiography and Doppler ultrasound of peripheral arteries. Allelic polymorphism of eNOs genes (T-786C) was assessed by polymerase chain reaction.
Results: In group I minor C allele and the C/C genotype of T-786S polymorphism of eNOs gene were significantly more prevalent than in group II: 56 (75.6%) versus 53 (42,1%), p=0,00001 and 22 (59,5%) versus 19 (30,2%), p=0,008, respectively. The presence of CAD in patients with PAD was associated with carotid atherosclerosis (RR 80%, p=0,0005), advanced stage of lower limb ischemia (RR 64%, p=0,03) and GFR below 60 mL/min/1,73m² (RR 72%, p=0,0043. According to the valid regression model we obtained (p<0,05), C allele of T-786C polymorphism of eNOs gene was responsible for 11.6% of risk of concomitant CAD in patients with PAD.
Conclusions: T-786C polymorphism of eNOs gene is one of the factors that determine presence of CAD in patients with PAD.