Background: Abnormalities of glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2) , were suggested with Celiac Disease (CD), by involvement of L-cells.
Aim of study : to explore the difference in ; duodenal expression of GLP1 Receptors and plasma concentrations of GLP-1 and GLP-2 during a carbohydrate stimuli between patients with naïve celiac disease (CD), 6 months post gluten free diet (GFD), and controls.
Methods: Twenty eight pediatric patients undergoing endoscopic gastroscopy agreed to add duodenal samples for GLP1-R and GLP2-R. Patient was included if was diagnosed as normal gastroscopy with negative antibodies or as CD, with positive antibodies. All underwent oral glucose tolerance test (OGTT) . CD patients underwent a second OGTT after 6 months GFD.
Results: Twelve patients were diagnosed with CD , Marsh 3, 7 males, mean age 9.2±2.5 years. Ten were in control group, 5 males, mean age 12±4 years, (p=0.14). Similar clinical, body composition and glycemic parameters were observed in both groups. Secretion pattern of glucose demonstrated a peak after 15-30 minutes , C-peptide peak level after 30-60 minutes, similar in all groups . Similar secretion pattern of GLP1 and GLP2 were detected in all groups, with a peak level after 15 minutes. The expression of GLP1 - R and GLP2 –R in duodenal tissue , as expressed by mRNA ratio to GAPDH , was similar between groups.
Conclusions : our study revealed no significant difference in pre as well as post-prandial secretion pattern and gut receptor expression of gut hormones, GLP-1 and GLP-2.
Acknowledgments: The study was supported in part by the HIPAK grant (MR, 2012).
