Sperm DNA Fragmentation Index Does Not Correlate the Rate of Blastocyst Aneuploidy in Autologous IVF Cycles
2IVF Unit, Tel Hashomer, Sheba Medical Center
3Pinchas Borenstein Medical Leadership Program, Tel Hashomer, Sheba Medical Center
4Obstetrics and Gynecology, University of Toronto
Introduction: High sperm DNA fragmentation Index (DFI) is related to male infertility and was previously suggested to be associated with poor outcome after IVF.
Aim: To determine if sperm DFI impacts blastocyst aneuploidy rate or morphological grade.
Materials & Methods: In this retrospective clinical study we included infertile couples in whom DFI was tested prior to IVF-Preimplantation Genetic Screening (PGS) by array Comprehensive Genomic Hybridization (a-CGH).
Results: The current study included 134 couples who underwent 177 IVF-ICSI-PGS cycles between January 1st, 2014 and March 31st, 2016. Group 1 (DFI>30%) encompassed 27 couples who underwent 35 cycles; Group 2 (DFI 15-30%) included 43 couples and 58 cycles; group 3 (DFI<15%) included 64 couples and 84 cycles. Male partners within group 1 were older (45.2 compared to 40.6 and 38.4 years respectively, p<0.05), had higher BMI (32.4 compared to 26.6 and 25.8 respectively, p<0.05) as well as lower sperm count and motility (45*106/ml and 35.5%, respectively) compared to groups 2 (61.8*106/ml and 46.6%, respectively) and 3 (77.1*106/ml and 55.2%, respectively, p<0.05). Female and embryo development parameters were similar. Total numbers of biopsied blastocysts were 95, 135 and 175 in groups 1, 2 and 3, respectively. PGS revealed comparable euploidy rate of 46-48% in all groups, with a similar morphological classification. Using logistic regression, both male and female ages were associated with blastocyst euploidy (OR 0.95, CI 0.93-0.98, p=0.001 and OR 0.91, CI 0.85-0.97, p=0.006, respectively).
Conclusions: We found no correlation between sperm DNA fragmentation and either blastocyst euploidy rate or morphological grading.
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