Rotavirus Infection in Children: Burden and the Dominant Genotype in 2014-2015 Years, Kiev, Ukraine
2Pediatric Infection and Immunology Department, Pediatric Infection Department, Shupyk National Medical Academy of Postgraduate Education, Pediatric Clinical Hospital #1.
3Pediatric Infection and Immunology Department, Pediatric Infection Department, Shupyk National Medical Academy of Postgraduate Education. Pediatric Clinical Hospital #1
4Pediatric Infection Department, Pediatric Clinical Hospital #1
Background: Rotavirus (RV) is the most common cause of severe gastroenteritis (GE) in infants and young children worldwide. There is no reliable epidemiological information on RV gastroenteritis (RVGE) in Ukraine. Rotavirus infection (RVI) is a vaccine preventable. To introduce universal vaccination, data on the burden of RVI and the dominant genotype must be collected.
Objective: To estimate the disease burden of RVGE and assess the relationship between the RV dominant genotype and the severity of clinical manifestations in Ukraine children up to 5 years old.
Methods: Hospital surveillance was conducted by using a WHO standard protocol. We performed an observational hospital based study which was conducted at Pediatric hospital #1 Kyiv, Ukraine during 2014-2015 years. The disease severity was estimated by score scale for clinical manifestations of RVGE assessment of Vesikari. Detection of RV antigen in feces was carried out by ELISA. RV genotyping was performed by RT-PCR.
Results: Among the 367 samples studied stool RVI was confirmed in 181 (49.3%). Genotyping was performed in 123 positive samples.
The following genotypes were revealed: G1P[8]-12.2%(15/123); G2P[4]-2.44%(3/123); G2P[8]-0.81%(1/123); G3P[8]-3.25%(4/123); G4P[8]-73.98% (91/123); G9P[8]-4.07%(5/123); G2[NT]-1.63%(2/123); NT[P8]-0.81%(1/123); NT[NT]- 0.81%(1/123). The mean number of points on a scale Vesikari: G1P[8]-15(3,04); G2P[4]-12(1,41); G2P[8]-15; G3P[8]-13.25(1,48); G4P[8]-14.68(2,56); G9P[8]-15.4(1,5); G2[NT]- 12(2); NT[P8]-13; NT[NT]-12. The number of points which is typical for moderate course met only with genotypes G1P[8]-6.67%(1/15) and G4P[8]-4.4%(4/91).
Conclusions: RVI is the main cause of hospitalization in children
Burden of RVGE, according to data about circulating genotypes justify the introduction of vaccination in Ukraine.
Key words: children, rotavirus, burden, genotype.
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