The Effect of Antiplatelet P2Y₁₂ Receptor Blockers on Angiogenic Properties of Vascular Endothelial Cells in Vitro

Introduction:Recent studies on P2Y₁₂ receptor blockers indicate the potential impact of these drugs not only to reduce cardiovascular outcomes, but also in tumor therapy by inhibiting platelet-endothelium reaction.
Objective: Endothelial cell exhibits P2Y₁₂ receptor on its surface, so it will also respond to antiplatelet drug, therefore we evaluated the impact of P2Y₁₂ receptor blockers on angiogenic properties of cultured endothelial cells in vitro.
Methods: HUVECs line EA.hy926 were exposed 24 h to the culture medium supplemented with anti-platelet drugs, in concentrations detected in the blood during standard therapy, with loading doses (LD) and clinically doses (CD) (ticagrelor - LD: 2 µM, CD: 1 µM, prasugrel - LD: 2 µM, CD: 0.3 µM, clopidogrel LD: 0.4 µM, CD: 0.04 µM). Cell proliferation wes measured using MTT test. Length of tube formation was measured using microscope Axio Observer D1 software. Cytokines in culture medium were tested using ELISA tests.
Results (mean ± SD) are presented in the Table
Conclusions: LD when compared with CD seems to have a stronger modifying effect on the angiogenic properties of the endothelium. Of the three tested drugs, Ticagrelor has the potent modifying effect on angiogenic properties of endothelial cells.