14C Myiristic Acid and 3H Cholesterol Uptake in Aging Rat Aorta Andheart Muscle.the Impact of Serum Factors from Dyslipidemic Patient

High levels of serum cholesterol represent an important risk factor for cardiovascular pathology.

The aim of this study was concerned with pointing out modifications in 3H Cholesterol as well as in 14C Myristic acid ( a common saturated fatty acid with the molecular formula CH3(CH2)12COOH), uptake in rat aorta and heart of different ages under the influence of serum factors from patients with lipid metabolic disorders.
Material and methods:aorta and rat heart ventricle of different ages have been incubated with 3H Cholesterol and 3 H Myristic acid in the presence of serum from male and female patients with dyslipidemia. The radioactive uptake of 3H Myristic acid and 3H Cholesterol was measured with a Beta Berhold Scintilation Counter.
The results have been statistically processed using Student “t” test. The differences relatively small between young and old animals concerning 14C Myristic acid uptake represent a proof that fatty acids with a monocatenary structure are major implicated in processes related with lipids uptake in the vascular wall and in heart muscle. 3H Cholesterol uptake is increased in young rat heart incubated with serum from female patients with dyslipidemia, while in aorta 3H Cholesterol uptake is net elevated when the incubation of aorta has been done in the presence of serum from male patients with dyslipidemia. In aging rat heart there is a significant increase in 3H Cholesterol uptake in the presence of serum from male patients with dyslipidemia, but in aging aorta, the 3H Cholesterol uptake is elevated in presence of serum from female patients with dyslipidemia.
Conclusion: the influence of human serum from patients with dyslipidemia upon 14C Myiristic Acid and 3H :Cholesterol uptake in rat aorta and heart of different ages accounts for the existence of serum factors which can modulate this process which depends upon age, gender and pathological state of donors.