Possibility of Determination Coagulation System Activation on Basis Expression of Tissue Factor

Background: In this part of the study, where we determined the causes of endothelial dysfunction at extracorporeal circulation , we focused on the role of tissue factor (TF) in the activation of these pathophysiological processes.
Recent findings attribute a significant part of the activation of coagulation creation of autoantibodies. Once this mechanism is activated, the antibodies induce activation of platelets and expression of tissue factor (TF, CD142) on monocytes and vascular endothelial cells.
Methods: We have proposed a monitor activation model of the coagulation system in endothelial dammage using TF expression on monocytes by flow cytometry, simultaneous determination the TF-induced thrombin generation in plasma and determination of platelet activity by measurements of aggregation. To determine expression of tissue factor (CD142) on monocytes, we proposed a method of multicolor flow cytometry using anti CD45 PerCP, anti CD14 APC, anti CD16b FITC, and anti CD142 PE antibodies and the corresponding isotype controls.
Results: We verified the model on patients who undergo cardiac surgery with the use of cardiopulmonary bypass, which is a high risk of endotheliel dammage.
Conclusions: We demonstrated complete inhibition of TF expression on monocytes and a significant reduction of thrombin generation in plasma.
Supported by grant LF-2017-007 and MH CZ – DRO (FNOl, 00098892)