Functionality of HDL and Its Associated Enzymes is Associated with HDL Subfraction Distribution in Coronary Artery Disease (CAD) Subjects - ICCAD 2017 - The 12th International Congress on Innovations in Coronary Artery Disease , 15-17 October, 2017 Venice, Italy

Himani Thakkar ¹ Vinnyfred Vincent ¹ Ambuj Roy ² Sandeep Singh ² R Lakshmy ³ Archna Singh ¹

¹Department of Biochemistry, All India Institute of Medical Sciences
²Department of Cardio-Thoracic Science Centre, All India Institute of Medical Sciences
³Department of Cardiac Biochemistry, All India Institute of Medical Sciences

Introduction: HDL sub fraction distribution and functionality are key factors for its anti atherogenic property.
Aim: This study aims to investigate correlation of HDL antioxidative activity and its associated enzymes with HDL sub fraction distribution.
Methodology: Subjects with CAD (N=65; 52±10yrs) and control subjects (N=50; 49±11yrs) were recruited after obtaining informed consent. Antioxidative functionality of HDL obtained by precipitation was measured by a dihydrorhodamine (DHR) based fluorescent cell free assay. HDL sub fraction distribution was assessed by single and double precipitation methods. Lecithin cholesterol acyltransferase (LCAT) and PON-1 activity were also compared.
Results: Compared with control, CAD subjects had decreased HDL-C as compared to controls; increased HDL2/3 ratio (1.138±0.048 vs 1.37±0.054) which showed positive correlation with antioxidative activity (P= 0.0179) and PON-1 activity (P=0.0065). Significant correlation was observed between Antioxidative activity and PON-1 activity (P=0.0067). From the correlation analysis, it was determined that LCAT activity had positive correlation with antioxidative activity (P=0.0257) and HDL-2 subfraction (P=0.049).
Conclusion: Decreased PON-1 and LCAT activity is likely to contribute to impaired HDL antioxidative activity. The decrease of enzyme activities is associated with altered HDL sub fraction distribution in CAD subjects.