DERMADYN-Dermatophytes detection test


Inbal Binsky 1 Marie-Jeanne Carp 1 Keren Ben-Zion 1 Maya Goshen 1 Noam Maisler 1 Rina Segal 2
1Molecular, Dyn R@D Ltd., Migdal Haemek, Israel
2Department of Dermatology, Rabin Medical Center, Bellinson Hospital, Petach Tikva, Israel

Overview-

A real time PCR- based multiplex application for fast, reliable, ready to use and high throughput in vitro diagnostic of dermatophytes. This multiplex PCR system enables 7 different dermatophytes types detection within 3 hours. The test contains two ready to use mixes for detection of 7 most common dermatopytes types:

[ 1 ] Trichophyton tonsurans

[ 2 ] Trichophyton violaceum

[ 3 ] Trichophyton rubrum complex

[ 4 ] Trichophyton mentagrophytes complex

[ 5 ] Microsporum canis

[ 6 ] Microsporum gypseum

[ 7 ] Epidermophyton floccosum

and Internal Control.

Specimen-

This test is for use with extracted DNA from skin scale specimens, plucked

hair, nails, culture and swabs of human origin.

Workflow-

Sample collection-sample lysis in lab-DNA purification manually or automated-add DNA and running Real

time PCR-results automatic analysis.

Clinical validation results-

A total of 220 samples were examined. Acorrelation of 86.3% (190/220) was found between the methods.

Cross reactivity detection- reference species from CBS

100% detection of 7 dermatophytes types. aditional detection of Microsporum audouinii Gruby

Test detection

1

CBS 358.93 Epidermophyton floccosum

2

CBS 161.69 Arthroderma gypseum

3

CBS 289.86 Trichophyton rubrum

4

CBS 319.31 Trichophyton violaceum

5

CBS 132.88 Microsporum canis

6

CBS 572.75 Trichophyton mentagrophytes

7

CBS 219.32 Trichophyton tonsurans

8

CBS 134.66 Trichophyton verrucosum

9

CBS 335.32 Trichophyton schoenleinii

10

CBS 404.61 Microsporum audouinii Gruby

11

CBS 511.73 Trichophyton erinacei

12

CBS 318.56 Trichophyton mentagrophytes

13

CBS 135.33 Acremonium strictum

14

CBS 287.95 Aspergillus fumigatus Fresen

15

CBS 467.48 Scopulariopsis brevicaulis

16

CBS 562 Candida albicans