Additional function of KaeA, the subunit of KEOPS/EKC complex?


Joanna Gawlik 1 Michał Koper 2 Piotr Węgleński 3,4 Agnieszka Dzikowska 2,3
1College of Inter-Faculty Individual Studies in Mathematics and Natural Sciences, University of Warsaw, Warsaw, Poland
2Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
3Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
4Centre of New Technologies, University of Warsaw, Warsaw, Poland

Kae1p, the subunit of KEOPS/EKC complex, is a highly conserved (Galperin and Koonin 2010), metal-binding protein belonging to the ASKHA (Acetate and Sugar Kinase, Hsp70 chaperone proteins and Actin) protein superfamily (Mao et al., 2008). It has been shown that Kae1p participates in the universal tRNA modification (t6A) (Perrochia L. et al, 2013). However, several published results suggest that Kae1p may also participate in other cellular processes. Both in S. cerevisiae (Kisseleva-Romanova et al, 2006) and in human cells (Costessi et al., 2012), participation of the KEOPS/EKC complex in transcription was suggested. Our published (Dzikowska et al., 2015) and unpublished results (i.a. NGS transcriptomic analysis of the kaeA mutants versus kaeA+ strain, KAEA cellular localization studies and t6A modification studies in kaeA mutants) suggest that also in the model filamentous fungus, Aspergillus nidulans, Kae1p may participate not only in t6A modification but also in regulation of transcription.

Bilbiography:

Dzikowska A. et al, Gene 573 (2015) 310–320

Galperin M.Y. and Koonin E.V. Trends Biotechnol. 28 (2010) 398–406

Mao D.Y. et al, Molecular Cell (2008) 32, 259-275

Perrochia, L. et al, Nucleic Acids Res. (2013) 41, 1953–1964.

Srinivasan, M. et al, EMBO J. (2011) 30, 873–881