Ascospore-specific gene expression analysis in Aspergillus nidulans
Developmental process and spore formation in a homothallic model filamentous fungus Aspergillus nidulans is environmentally and genetically regulated. Although asexual spores or conidia differentiation is well-characterized by analyzing important genes for controlling orchestrated developmental pathways, including the brlA gene-mediated conidiophore and conidia morphogenesis, a few genes have been elucidated for playing an important role in sexual development and ascospore formation. The nsdD, nsdC and veA genes are responsible for regulation of sexual and asexual developmental processes. Unlike conidia, however, physiological and genetic studies of ascospores are remained to be characterized. To know more about the ascospores biology, we performed RNA-seq analysis from from A. nidulans conidia and ascospores RNA samples. Comparative analysis of transcription profiles of conidia and ascospores revealed many genes that are expressed differentially in both spores. Detailed investigation of the differentially expressed genes is in progress.