Proteomic analysis of temperature dependent total proteins from Trichoderma guizhouence NJAU4742 by using rice straw as sole carbon sources under solid-state culture condition

Tuo Li 1,2 Xiaohui Meng 1,2 Xing Chen 1,2 Lei Ma 1,2 Liu Dongyang 1,2 Qirong Shen 1,2
1Jiangsu Key Laboratory for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, China
2Solid Organic Waste Resource Utilization, Jiangsu Collaborative Innovation Center, Nanjing, China

Trichoderma guizhouence NJAU4742 is a mesophilic filamentous fungus isolated from compost, which plays a vital role in prompting plant growth through acting as plant growth promoting and owns a capacity to degrade various agricultural wastes. The metabolic rate is inhibited by the parameter of temperature, especially the ability of decomposing lignocellulosic materials, however, the biological mechanism is not clear. In this study, various extracellular and intracellular proteins synthesized by T. guizhouence NJAU4742 by using rice straw as sole carbon sources were explored by using the quantitative proteomic approach Sequential Windowed Acquisition of all Theoretical fragment ions (SWATH) with Data Dependent Acquire (DDA) mode for relative quantification. The results indicated that 1464 proteins were all identified in all the treatments (cultured at 20 oC, 28 oC and 37 oC) including many intracellular regulatory proteins and extracellular hydrolytic enzymes (cellulases, hemicellulases, lignin-degrading enzymes, proteases), protein-translocating transporter, and hypothetical proteins by using rice straw as sole carbon sources. Quantitative SWATH and protein network interaction analysis results showed that ADP ribose pyrophosphatase may be the critical factors to effect the metabolic rate of T. guizhouence NJAU4742 during the biodegradation process at different temperature. Therefore, we discuss here the possible biological mechanism how temperature effect the lignocellulose decomposition by T. guizhouence NJAU4742 and the optimization of lignocellulosic biomass hydrolysis by T. guizhouence NJAU4742.