Genetics of monokaryotic fruiting in Schizophyllum commune

Ioana Marian 1 Florian Hennicke 1,2,3 Luis G. Lugones 1 Han A.B. Wosten 1 Robin A. Ohm 1
1Department of Biology, Utrecht University, Utrecht, Netherlands
2Genetics and Genomics of Fungi, Senckenberg Gesellschaft für Naturforschung, Frankfurt, Germany
3Evolution and Diversity, Institute of Ecology,Goethe-University, Frankfurt, Germany

In the mushroom-forming basidiomycete Schizophyllum commune the fruiting bodies are generally only formed by dikaryotic individuals. However, monokaryotic fruiting does occur in some strains. We have identified a monokaryotic strain that forms mushrooms when grown in the light. Interestingly, this trait showed mendelian inheritance upon crossing with a reference strain that does not produce monokaryotic fruiting bodies, suggesting that only a single gene/locus is involved. In order to identify this gene we are planning to perform a bulked segregant analysis (BSA). Furthermore, the monokaryotic fruiting bodies were subjected to histological analysis to determine differences and similarities with normal dikaryotic fruiting bodies.