Trametes versicolor and Schizophyllum commune as early sapwood colonizers on wood

Amjad Zia Andrzej Majcherczyk Ursula Kues
Molecular Wood Biotechnology and Technical Mycology, Universoty of Goettingen, Goettingen, Germany

Schizophyllum commune and Trametes versicolor are early sapwood fungi in the decay of wood of broadleaf trees. In nature, they can be observed together on branches of living trees, on freshly fallen dead wood and also on cut stems. However, S. commune is a weak degrader with features in between white and brown rot and may make use of wood extractives and other easy accessible compounds. T. versicolor in contrast is an aggressive white rot. In decay tests with beech wood, up to 52% reduction in sapwood weight of beech wood has been recorded by T. versicolor and at most 5% by S. commune. In our studies, we address the behavior of the two fungi in single and dual culture through analyzing the proteomes of the two fungi grown on beech sapwood particles in light and dark conditions. In dual culture on wood, S. commune appeared to be the first invader. Later, T. versicolor grows over the wood with S. commune. In the dark, T. versicolor grew faster over the wood with S. commune as compared to in light. Light induces in T. versicolor production of dense surface mycelia and also aggregated structures as possible step in fruiting body development. Secretomes and intracellular proteomes are isolated from wood cultures of different age. We first analyzed the secretomes of 10 and 28 days old T. versicolor dark grown cultures. Typical enzymes (different types of cellulases, peroxidases; in total 77 enzymes) in wood decay were shared between the samples. However, the early decay samples contained a higher number of laccases with potential effects on lignin degradation while chitinases with possible functions in reuse of aged mycelium were unique to the older samples.

We appreciated support by the Ministry of Lower Saxony (MaFoHolz PhD program) and by the DFG (DFG-GZ: INST 186/1085-1 FUGG).