NOVEL POLYMER MATRIXES FOR CONSTRUCTION OF AMPEROMETRIC BIOSENSORS

Taras Kavetskyy ^1,2 Oleh Smutok ³ Mykhailo Gonchar ³ Roman Leshko ¹ Yuliia Kukhazh ¹ Mariana Kravtsiv ¹ Khrystyna Zubrytska ¹ Oksana Mushynska ¹ Ondrej Šauša ⁴ Helena Švajdlenková ⁵ Tamara Petkova ⁶ Victor Boev ⁶ Vania Ilcheva ⁶

¹Solid State Microelectronics Laboratory, Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine
²Department of Applied Physics, The John Paul II Catholic University of Lublin, Lublin, Poland
³Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
⁴Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovakia
⁵Polymer Institute, Slovak Academy of Sciences, Bratislava, Slovakia
⁶Institute of Electrochemistry and Energy Systems, Bulgarian Academy of Sciences, Sofia, Bulgaria

Development of a novel nanocomposites and polymers suitable for construction of biosensor with improved operational parameters is an important field in sensor technologies. For the first time, novel polymer matrixes based on the urea-silicate or ureasil composites [1-5] were tested for immobilization of laccase and construction of amperometric biosensors [6]. The biosensor characteristic such as sensitivity of the bioelectrode was determined and analyzed for the ureasil-chalcogenide glass composite, ureasil, and ureasil-chalcogenide glass composite with incorporated silver nanoparticles (NPs) synthesized by high-dose 30 keV Ag ion implantation. A very high sensitivity of amperometric biosensor with ureasil-chalcogenide glass composite was established and a well expressed influence on the sensor’s characteristics by polymer matrix and Ag NPs was detected [6]. In the present work, a correlation between the network properties as revealed by low-temperature positron annihilation lifetime spectroscopy, and swelling experiments [7] and biosensor characteristics of pure ureasil and ureasil-chalcogenide glass composite of different pre-history (fresh and aged during one year) is established. The observed findings could be further used for improvement of operational parameters of laccase-based amperometric biosensors, which may have potential for monitoring the level of pollution of wastewater containing xenoestrogens.

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[3] V.I. Boev et al., J. Sol-Gel Sci. Technol. 41, 223 (2007).

[4] T. Kavetskyy et al., Phys. Status Solidi C 9, 2444 (2012).

[5] T. Kavetskyy et al., Mater. Sci. Forum 733, 171 (2013).

[6] T. Kavetskyy et al., J. Appl. Polym. Sci. 134, 45278 (2017).

[7] T. Kavetskyy et al., Acta Phys. Pol., A (in press).