Dual Fuel Assisted Bistable Non-Enzymatic Networks

Indrajit Maity maityindrajitchem@gmail.com Nathaniel Wagner Rakesh Mukherjee Rivka Cohen-Luria Gonen Ashkenasy
Chemistry Department, Ben-Gurion University of the Negev, Beer-Sheva, Israel

Cellular memory circuits usually rely on bistable phenomena to convert a transient signal or stimulus into a sustained response which can control the biological processes like cell division, apoptosis and cellular differentiation. Since cells operate far from chemical equilibrium, they often present dynamic nonlinear behavior such as oscillations, bifurcation and multistationarity, to the extent that these functions have been suggested as prerequisites of life.1 However, the minimal requirement and mechanism of bistable reaction network does not yet properly understandable. A bistable system is characterized by simultaneously reaching two stable steady states depending on control parameter in bifurcation zone (Figure 1A). Herein, we emphasize a reversible self-replicating reaction between an electrophile E and nucleophile N to produce a replicator R in coiled coil peptide networks2 which leads to one of two distinct steady-state (SS) concentration distributions depending on initial replicator concentrations (Figure 1B). Here, bistability arises because of the non-linear positive feedback and non-symmetric reversible processes.3,4 We have preferred some environmental perturbations on the bistable system to precisely map the overall bistable zone along the parameter spaceses. We map the overall bistable zone along the several parameters space like temperature,concentration of total materials, thiol and guanidine hydrocholoride. Furthermore, this system shows the dual fuel dependency for effective replication and hence to invoke the bistability. These present an unprecedented far from equilibrium phenomena with non-enzymatic catalysis which may be more responsible in designing of biomimetic networks and highlighting the probable roles in early evolution as well as can display their significance in other branches of science.

Figure 1: (A) The bistable system. (B) Bistable behavior observed along R thiodepsipeptide equilibration experiments.

References

  1. S. Tsokolov, Astrobiology, 2010,10,1031
  2. Z.Dadon; M. Samiappan; A.Shahar; R. Zarivach; G. Ashkenasy; Angew. Chem. Int. Ed. 2013, 52, 9944; Angew. Chem.2013, 125, 10128.
  3. R. Mukherjee; R. Cohen-Luria; N.Wagner; G. Ashkenasy; Angew. Chem. Int. Ed. 2015, 54, 12452.
  4. N. Wagner; R. Mukherje; I. Maity; E. Peacock-Lopez; G. Ashkenasy; ChemPhysChem 2017, 18,1842.








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