Wound Healing Assay of a Cell Monolayer Containing Two Types of Cells Labeled with Photothermal Gold Nanoparticles
Department of Biomedical Engineering, Tel Aviv University
Scratch wound healing assay is a convenient and cost-effective method for analysis of cell migration in vitro. The assay involves first creating a “scratch” in a cell culture monolayer, then capturing the images at the beginning and at constant intervals during cell migration to full closure of the gap, and finally comparing the images to quantify the migration rate of the cells. However, it is not trivial to perform this assay when two types of cells are available in the culture.
Our aims are to evaluate and compare the migration rate of two different types of cells by creating a “scratch” on the confluent cell monolayer using labeling with gold nanoparticles.
We performed a scratch wound healing assay on a confluent cell monolayer that contains two types of cells. The cells were labelled with plasmonic nanoparticles for evaluation of cell migration and detected by using photothermal interferometric phase imaging.
Shir Cohen Maslaton
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