Design and Construction of Three Dimensional in Vitro Model for Investigation of Ovarian Carcinoma Progression Mechanisms - ISMBE - Biomedical Engineering, International Convention Center Haifa 22.2.2018

Aharon Shmuel Baskin ^1,2 Reuven Reich ² Ben Davidson ^3,4 Tali Tavor - Reem ¹

¹Dep. Of Pharmaceutical Engendering, Azrieli College of Engineering
²Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem
³Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital
⁴The Medical Faculty, University of Oslo

Introduction: Ovarian carcinoma presents a significant challenge both in designing effective drug treatment and in understanding cellular processes during disease progression, especially during the transition from a solid form of the tumor to a detached cellular spheroid form, in effusion. Emerging evidence shows the various functions of Sphingolipid-1-phosphate (S1P) as a potent regulator of cancer progression. Cancer research is currently limited by the lack of an appropriate in vitro model of the tumor microenvironment. An ideal model must take into account the various components of this microenvironment.

Aims: Design To establish a 3D in vitro culture system of OC cells to mimic the 3D metastatic tumor microenvironment.

Methods: Comparing between distribution and levels of S1PR mRNA in the over 250 real samples from the different sites of OC with those in cell lines,were grown in the various conditions: traditional 2D, spheroids, alginate scaffolds with and w/o perfusion bioreactor.

Results and Conclusions: Use of the Perfusion Bio Reactor together with the alginate scaffolds achieved similar S1PR expression levels to the in vivo. Growing ES-2 cell line cells in those conditions seems to be the most suitable for the in vitro modelling for the S1PR system.