Genetic Oscillator Circuit without Delay – Design and Investigation

Genetic synthetic engineering is a discipline that targets the microlevel and genes control circuits. Understanding genetic circuits is required for deciphering design principles of system biology, diagnostic, therapeutic and biotechnological applications. In order to do that, a good understanding of biology, control theory, MEMS chip building and microfluidics¹ is required.

we fabricate a harmonic constant oscillator with secondary negative feedback (NFB) and no delay. Secondary NFB – we will obstruct through a competitive process the RNA polymerase path rather than stop the promotor activation or degrade the protein (figure 3) thus achieving phase change without delay. This enables us to not enter a delay to the dynamics.

In this research we want to build an oscillatory circuit without delay which have constant inner parameters. A circuit with the minimum strains and processes will be faster and shall allow better understanding of the biology. By controlling its substance flow, amount of e-coli and signaling between types of e-coli we will be able to obtain oscillation frequencies as we desire. This system is not just tuning of processes by synchronize clocks, but a living computing device that reacts to changes in its surrounding. Such system will be a building block for bio – machine chips.

Plasmids and bacteria shall be prepared per established lab protocols.
Plasmids shall be sequenced by macrogen.
The chip will be designed by guidelines given in “clean rooms” course and J. Hasty handbook for designing a microfluidics chip.

We have managed to show that theoretically a biological level can exist on the threshold of stability in genetic engineered E.coli.
We are now testing the protein experssion of the plasmids and chip geometery