What is the Impact of BMI on Lipids Accumulation in Cumulus and Granulosa Cells?

Introduction

It is well established that obesity affect fertility treatments. Moreover, obesity enhance lipids storage in cells other than adipocytes including cumulus and granulosa cells, and damage steroidogenesis.

Aim

To examine the effect of BMI on lipid content in human cumulus and granulosa cells, and to correlate it with fertility treatments outcomes.

Methods

During OPU Granulosa and cumulus cells were collected, fixed and stained with Nile red to evaluate lipid droplet content by florescent microscopy, and by flow cytometry. Additionally, blood sample and the follicular fluid were analyzed.

Results

Data was collected from 41 women; 26 with low BMI ( BMI < 25) , and 15 with high BMI (BMI > 30). We found no statically significant difference in conception rate and lipid content between the two BMI groups. In low BMI group, less embryos were transferred and more were vitrified (1.2±0.9 vs. 1.8±0.8; P=0.04 and 2 vs. 0; P=0.03; respectively). The follicular and serum CRP levels were significantly lower. Comparing the pregnant and non-pregnant patients, no difference was observed in basic characteristics and cycle outcome. However, we demonstrated lower accumulation of lipids in the cumulus and granulosa cells in those who conceive compared to those who did not conceive (Cumulus cells- 17533 vs. 42000; P=0.001 and granulosa 14771 vs. 24121; P=0.03). Concomitantly, follicular and serum estradiol and follicular triglycerides were significantly higher in the pregnant group.

Conclusion

BMI per se didn’t influence pregnancy rate. However, accumulation of lipids in the cumulus and granulosa demonstrated adverse effect on pregnancy.