To improve plants tolerance to abiotic stress it is important to understand the various mechanisms that plant use to avoid stresses during growth and development. The study of transpiration, water and nutrient uptake during abiotic stress in the root zone is hindered because of the hidden nature of roots. Modified aeroponic system was used and proven to be a unique device for the potential evaluation of the effect of salinity, nitrogen and water uptake on the growth and development of tomato plants. Daily transpiration, rate of water and NO3 uptake, cumulative transpiration and relationship between water and NO3 uptake were estimated. The modified aeroponic system were proven to be; a unique device for investigating effects and response of plants grown under various environmental stresses within a short period of time; continues monitoring and evaluating of root growth and estimation of transpiration, water and nutrient uptake which otherwise cannot be done in the soil growth conditions.