MOLECULAR IMPRINTING OF BIOMOLECULES WITHIN NANO PORES

Mesoporous nanoparticles, which possess highly ordered narrowly distributed nanopores, have already gained enormous attentions in catalysis, separation and sample treatment. Molecular imprinting within mesoporous nanoparticles could further endow this type of materials with desirable properties including affinity and specificity towards target compounds and thereby could enable promising applications. However, highly efficient molecular imprinting within mesoporous materials remained challenging. Recently, we have developed a new strategy, called dual-template docking oriented molecular imprinting (DTD-OMI), for facile and highly efficient imprinting within mesoporous silica nanoparticles (MSNs).^[1] As compared with the synthesis of non-imprinted MSNs, DTD-OMI did not require additional step, but provided ultrahigh imprinting efficiency and excellent binding properties. Using this strategy, we prepared phosphate-imprinted MSNs and demonstrated them as an ideal sorbent for selective enrichment of phosphopeptides for highly efficient mass spectrometric analysis of protein phosphorylation.^[2] More recently, we further synthesized Amadori compound-imprinted MSNs for highly specific and efficiently extraction of Amadori compound of interest from complex samples.^[3] In this talk, we will mainly introduce the DTD-OMI approach for molecular imprinting of biomolecules within nano pores. Particularly, we will introduce the imprinting of oligodeoxyribonucleotides within MSNs for the construction of DNA ligase-mimicking nanoreactors, which can link two short single-strand DNA into a long one.

References:

1. Yang Chen, Xinglin Li, Danyang Yin, Daojin Li, Zijun Bie, Zhen Liu. Chem. Commun., 2015, 51, 10929-10932.
2. Yang Chen, Daojin Li, Zijun Bie, Xinpei He, Zhen Liu. Anal. Chem. 2016, 88, 1447–1454.
3. Xianghua Pan, Xinpei He, Zhen Liu. Anal. Chim. Acta 2018, in press.