Neonatal Sepsis and Blood Culture

Introductions : Infections is an important cause of morbidity and mortality in the neonatal period , particulary those born preterm and very low birth weight.(VLBW). Early diagnosis of neonatal sepsis is challenging because clinical characteristics are non-specific and difficult to differentiate from those of non-infectious etiologies. Blood culture remains the gold standard for diagnosis of neonatal sepsis, but the rate of positivity is low, influenced by factors such as intrapartum antimicrobial administration and limitations in blood volume per culture that can be obtained in neonates.

Aim-Material The study was prospectivly conducted over a period of 12 mounts between 01.01.2015 to 31.12.2015.The purpose was to identifications the bacterial microorganisms caused neonatal sepsis in blood culture.Blood was collected from a peripheral vein, approximately 1 ml of blood.

Results:The present study included 71 newborn infants diagnosed with sepsis. Out of 653 infants admitted from 01.01.2015 to 31.12.2015., 427 were born at term(TNB ) and 227 were preterm infants (PTN ). They were divided into two groups: one comprised of at term newborn infants with proven sepsis - 32 cases (7,5%) and second group were preterm newborns with neonatal sepsis - 39 cases (17,3%). Blood culture was positive in 44 newborns ( 24 PTN and 20 TNB) with sepsis (62%). In the PTN, E. coli (25%) and Klebsiella pneumoniae (25%) were most often causes of neonatal sepsis, followed by Staphylococcus CoN (20.8%). In the TN, dominant causes were Staphylococcus aureus (25%) and Staphylococcus CoN (25.0% ), followed by E. Coli (10%) and SGB (5.0%).In the PTN dominant isolated pathogen in early-onset sepsis was E. coli , while in late onset sepsis were Klebsiella Pneumoniae and Staphylococcus CoN. TNB with early-onset sepsis, blood culture were positive (44.4%), and dominant bacteria with a slightly frequent presence of Klebsiella Pneumoniae and Staphylococcus CoN, but in the late-onset sepsis Staphylococcus Aureus was present mostly in admitted infants from home.

Conclusions:It is important to identifications the bacterial microorganismsims in our region,analysis of longitudinaltrends assist in the formulations of strategies to treat and prevent neonatal serious infections.