Isolation of Circulating Nucleic Acids for In-vitro Diagnostic Use

Circulating nucleic acids play an increasing role in obtaining diagnostic information in non-invasive prenatal testing (NIPT) and in non-invasive analysis of the genetic makeup of tumors (liquid biopsy). The performance of such assays is dependent on the efficient and complete extraction of circulating nucleic acids from blood samples. We recently developed a new CE-IVD version of the industry-leading QIAamp Circulating Nucleic Acid Kit for in vitro diagnostic use. It uses proven silica-membrane technology for isolation and purification of circulating, cell-free DNA and RNA from human blood plasma samples.

To improve the usability and processing time of the extraction process, we also developed an optional streamlined version of the circulating nucleic acid isolation protocol that reduces processing steps and time. Present data show that equivalent performance and excellent reproducibility as well as eluate performance in different down-stream applications is fully maintained in the updated protocol version.