Functional Features of the Duodenum in Children with the Syndrome of Bacterial Overgrowth in the Small Intestine - EAP 2019 Congress and MasterCourse Porto Palacio Hotel

V.P. Novikova ¹ M.M. Gurova ¹ A.P. Listopadova ¹ O.M. Tsekh ¹ E.Yu. Kalinina ² E.A. Orishak ³ A.N. Petrovsky ²

¹Laboratory of Medical and Social Problems in Pediatrics SIC, St. Petersburg State Pediatric Medical University, Russia
²Department of Pathological Anatomy, North-Western State Medical University named after I.I. Mechnikov, Russia
³Department of Medical Microbiology, North-Western State Medical University named after I.I. Mechnikov, Russia

Objective: To study the relationship between the functional state of the duodenum and its microbiocenosis in patients with duodenitis associated with Helicobacter pylori (HP) infection.

Materials and Methods: In our study, we included 60 adolescents aged 14 to 18 years with morphologically proven duodenitis. We detected HP by PCR in feces. Bacteriological examination of the duodenal contents extracted during endoscopy via the endoscope biopsy channel was performed. The activity of enzymes in duodenal biopsy specimens was determined according to the method of A.M. Ugolev and N.M. Timofeev.

Results: It was revealed that helicobacteriosis without syndrome of intestinal bacterial overgrowth (SIBO) did not affect the activity of the brush border enzymes. In case of combination of helicobacteriosis and SIBО we found it negative correlation with the activity of lactase (r = -0.489, p <0.05) and aminopeptidase M (r = -0.487, p <0.05). Combination of helicobacteriosis and giardiasis had a negative correlation with the activity of lactase (r = -0.33, p <0.05) and glycyl-L-leucine dippeptidase (r = -0.324, p <0.05), whereas the combination of helicobacteriosis with SIBО and giardiasis reduced the activity of all three enzymes: lactase (r = -0.432, p <0.05), aminopeptidase M (r = -0.461, p <0.05) and glycyl-L-leucine dippeptidase (r = -0.374, p <0.05). We have identified microorganisms that have a negative effect on the lactase activity: Actinomyces (r = -0.318, p <0.05), Staphylococcus species (r =-0.416, p <0.05), Pseudomonas aeriginosa (r =-0.435, p <0.05) Peptococcus species (r =-0.455, p <0.05), Candida albicans (r =-0.346, p <0.05), Enterococcus faecalis (r = -0.426, p <0.05). We found a positive correlation between the presence of lactobacilli in the small intestine and the activity of aminopeptidase M (r = 0.341, p <0.05).

Conclusions: The microbiocenosis of the duodenum in adolescents with HP-associated duodenitis affects the activity of the brush border enzymes.