CRISPR knockout in the red microalga Porphyridium sp.

The molecular genetics research of red microalgae lags far behind that of higher plants and animals, mainly because of lack of advanced tools. The major aim of this research is to develop a genetically modified red microalga Porphyridium sp. using the gene editing CRISPR/Cas9 tool. The Porphyridium sp. cells are encapsulated within a thick layer of amorphous sulfated polysaccharide. The sulfated polysaccharide was found to have various bioactivities, including anti-inflammatory, antioxidant and antiviral. The bioactivity is attributed to the sulfate groups. The CRISPR knockout of the model gene sulfotransferase, enzyme involved in the sulfation of this sulfated polysaccharide, will contribute to our understanding on the function of the sulfate groups and the bioactivities of the polysaccharide. Developing this technology will help to implement the technology in additional algal species.