The nuclear envelope (NE) serves as both a protecting membrane shell of the genome and an adaptable communication interface between the nucleus and the cytoplasm. In metazoans, the NE is physically supported by the nuclear lamina (NL), a meshwork of filaments formed by lamins and lamin- binding proteins, where Lamin B mainly provides elastic properties that allow significant deformation of the NE, while Lamin A confers deformation-resistant viscous stiffness to the nucleus. Moreover, the NL provides an anchoring platform for heterochromatin domains, so-called lamina-associated domains (LADs) and participate in genome organization. It is believed that lamins affect cell migration by changing the nuclear physical properties, while their chromatin binding in migrating cells has not been evaluated yet. Here, to determine genome-wide changes in chromatin association of Lamin B and Lamin A upon induction of cell migration, we performed ChIP-seq analysis on mouse melanoma cells. Significantly, in migrating cells Lamin B lost 60% of its LADs, Lamin A lost around 15% of them, while no new LADs were formed. Comparison of altered LADs position and migration-induced transcriptome changes did not identify any correlation between them. However, the lost LADs were mainly short ones that in most chromosomes were localized preferentially to only one chromosomal arm, leading to changes in the nuclear architecture in migrating cells. The consequences of these nuclear architecture changes in migrating cells will be discussed.