Many different types of nuclear bodies can be found in the nucleoplasm including 10-30 sub-nuclear structures called nuclear speckles. The function of nuclear speckles is debatable. Many types of splicing factors have been found to reside in nuclear speckles, as well as RNAs including long non coding RNAs (lncRNA). Although the functions of lncRNAs have long been unknown, in recent years they have been discovered to take part in a broad range of cellular processes ranging from chromatin modification to regulating mRNA stability and post-transcriptional processes. Altered functions of protein or RNA components of nuclear speckles have been associated with multiple diseases and rare disorders adding to the importance of determining its composition and the factors involved in their proper architecture and function. We are interested in the nuclear organization and retention of lncRNAs and their correlation to nuclear speckles as well their function in relation to the other nuclear speckle residents. To this end, we have been studying the MEG3 lncRNA, a nuclear retained transcript, and using a cell system in which this lncRNA can be followed in living cells, we have been investigating the function and dynamics on MEG3 in the nucleus.