Structural Proteomics Unit – From Gene to Protein 3D Structure

The Structural Proteomics Unit (SPU) is part of the Life Science Core Facilities (LSCF) at the Weizmann Institute. The SPU implements all steps in the pipeline from the gene to the 3D protein crystal structure. The center collaborates with and provides service for the scientific community and for clinicians by making available its expertise in DNA manipulation, protein expression, protein purification and protein structure determination and analysis. In addition, large quantities of purified proteins are produced for biochemical and biophysical studies. The SPU is constantly engaged in development of new methodologies, as well as in the implementation of state-of-the-art technologies to deal with challenging scientific questions.

The center uses Restriction Free (RF) and Transfer-PCR (TPCR) cloning methodologies. The strength of these methods lies in their simplicity and flexibility, which can be harnessed for a large spectrum of applications. These include cloning of multiple DNA fragments, insertion and/or deletion of specific DNA sequences, and single and multiple-site mutagenesis. The SPU utilizes several expression systems including bacterial system and three eukaryotic systems, such as yeast, insect and mammalian cells. Proteins are purified primarily by FPLC systems. In addition, we provide assistance in protein refolding and protein characterization. Protein- structure determination is performed primarily by X-ray crystallography. The SPU perform detailed comparative sequence-based-structure analysis of related proteins and correlate the protein structure to its function, biochemical findings and biological importance.