ILANIT 2020

ABSTRACT BY SPONSOR: New technologies for enhancing the efficiency of CRISPR-based cell engineering at scale

Kevin Holden
Head of Science, Synthego

Synthego has developed automated platforms for engineering immortalized cell lines and stem cells at scale, using the CRISPR-Cas technology. In order to improve the efficiency of genome engineering, new methodologies and technologies are required. We describe innovative technologies and methods for: optimizing and standardizing CRISPR editing methods; temporal control of CRISPR on and off-targeting; improved tools for predicting editing outcomes; methods for precisely manipulating edited cell populations.









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