Generation and Characterization of a Mouse Model for one Anastomosis Gastric Bypass Surgery
2Surgery, Hospital of the University of Pennsylvania
3Developmental Biology and Cancer Research, Hebrew University of Jerusalem
4Military Medicine and Tzameret, Hebrew University of Jerusalem
5Institute for Drug Research, Hebrew University of Jerusalem
Objective:
One Anastomosis Gastric Bypass (OAGB) surgery became a common bariatric procedure in recent years. In this surgery, the distal stomach, duodenum, and proximal jejunum are bypassed, leading to weight loss, improvement in metabolic parameters, and a change in hormonal secretion. We sought to generate and characterize a mouse model for OAGB.
Methods:
Mice fed for 26 weeks on a high-fat diet were assigned to OAGB, sham surgery, or caloric restriction and were followed for fifty more days on a high-fat diet. Physiological and histological parameters of the mice were compared during and at the end of the experiment.
Results:
OAGB-operated mice lost weight and displayed low levels of plasma lipids, high insulin sensitivity and rapid glucose metabolism compared with sham-operated mice. OAGB-operated mice had higher energy expenditure, higher levels of Glp1 and lower albumin than weight-matched calorie-restricted mice. There was no difference in the histology of the endocrine pancreas. The livers of OAGB mice had little hepatic steatosis, yet presented with a large number of phagocytic cells.
Conclusions:
The OAGB mouse model recapitulates many of the phenotypes described in patients that underwent OAGB, and enables molecular and physiological studies on the outcome of this surgery.