Oocyte eligible for Intracytoplasmic Sperm Injection (ICSI) are those that progressed through meiosis to MII. The remaining delayed mature oocytes can be injected, aiming to achieve more embryos and better chance to conceive. We aimed to assess the outcome of delayed matured oocytes, derive from either GV or MI, that reached maturity (MII) 24 hours following retrieval. The study population consisted of 362 women who underwent 476 IVF cycles. While fertilization rates were comparable between the sibling delayed mature oocyte group compared to injection on day 0 group (58.4% vs 62%, respectively, p=0.07), the top quality embryo rate per injected MII day 0 oocyte was significantly higher as compared to day 1 injected oocyte (57.5% vs 43.9% respectively, p