Anti Triple-negative breast cancer tumor effect, of an Ambrosia confertiflora herbal preparation

From prehistoric times to the present, plants have been employed as a source of medicine. The applications of plant-based natural products play a crucial role in primary healthcare practice worldwide. The general public has long considered natural product-based medicines effective and safe. Therefore, many pharmacological investigations are carried out on identifying and characterizing natural products to develop a novel therapeutic agent for treating several diseases, including cancer.

Ambrosia confertiflora is an important medicinal plant used in traditional medicine. This study aims to investigate the efficacy of the ethanolic extract of areal parts of A. confertiflora under in-vivo and in-vitro conditions. The extract is non-toxic to the experimental mouse and has no cytotoxic effect.

A significant difference in the size of triple-negative carcinoma tumors has been observed in mice model of breast cancer. In the treated mouse, the tumors were significantly smaller than the control. In addition, the tumors from the treated mouse had a large mass of necrotic cells as compared to the control mouse. In-vitro observations on macrophages show an increase of anti-inflammatory and anti-cancer cytokines, indicating the extract`s role against cancer cells. An RNA-SEQ on a row of triple-negative carcinoma cancer cells and a macrophage row of cells was conducted to test the extract`s effect on gene expression. The extract makes stimulation of macrophages to differentiate into anti-inflammatory and anti-cancer macrophages. In some ways, Macrophages cell therapy is similar to CAR T-cell therapy. Both transform or enhance immune system cells so they can effectively fight cancer