Running affinity measurements on the Proteon XPR36 – experimental guidelines.
Ilse Katz Institute for Nanoscale Science and Technology, Ben Guriun University, Israel
A major tool in protein and drug research is affinity measurements. Affinity is used to compare activity of a new modified protein or antibody to that of the w.t. molecule. One can also measure interactions between small molecules or nucleic acids to proteins thus helping to elucidate or define their biological role. There are several methods for measuring affinity: Surface Plasmon Resonance (SPR), Micro Scale Thermophoresis (MST), Isothermal titration calorimetry (ITC) and more. Here we show the guidelines for planning an SPR experiment using the ProtOn XPR36 (previously Biorad, now Biosensor Tools).