D-2-Hydroxyglutarate (D2HG) detection kit (# MAK320)

Metabolic diseases like rare autosomal disorder D2HG aciduria, and various cancers and neoplasms like Intra-hepatic cholangiocarcinoma (IHCC), have mildly to a high level of D-2-Hydroxyglutarate (D2HG). In addition, mildly elevated levels of D2HG can be found with mutations in the isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) genes.

Thus, the detection of elevated D2HG is an important biomarker for early diagnosis, prognosis, monitoring, and development of therapeutic strategies against these diseases.

In collaboration with Prof. Stefan Pusch from the German Cancer Research Center (DKFZ), we have developed the D2HG Assay Kit (#MAK320). This kit allows rapid and sensitive enzymatic assay for the detection of D2HG levels in various biological fluids: serum, urine, cell culture supernatants, and cell or tissue lysates. The assay, originally developed by Balss et al. is based on the oxidation of D2HG to a-ketoglutarate (aKG) by the enzyme (D)-2-hydroxyglutarate dehydrogenase (HGDH) coupled with the reduction of NAD+ to NADH. The amount of NADH formed is then quantitated by the diaphorase-mediated reduction of resazurin to the fluorescent dye resorufin.