Characterization of Biliary Atresia expression profile in cholangiocyte organoids

Biliary Atresia is a neonatal liver disease with extrahepatic bile duct obstruction and progressive liver fibrosis. The etiology and pathogenesis of the disease remain unknown. In this study, we examine the expression profiles of BA from biliary epithelial cells known as cholangiocytes using organoid technology which has become a reliable developmental biology tool for organ specific evaluation. In this research we focus on identifying the pattern of the disease using bulk and single cell gene expression data from different patients. In addition, we wish to determine the transcriptional profiles caused by toxin biliatresone, that has been shown to lead to BA and extrahepatic fibrosis.