New Collagenase Blend for Functional and Viable Cell Isolation from specific tissue dissociation

Tissue dissociation process is a crucial step in the release of functional and viable cells from animal tissues, with minimal impact on the cell’s integrity. Collagenase enzymes are the main reagent used for the tissue dissociation process due to their ability to break down the native collagen that holds animal tissues and thus release the intact cells. Researcher’s challenge is to find the optimal conditions for complete and intact cell isolation from the tested tissue sample. Most of the protocols require multistep process as adding a second digestion enzyme to improve the tissue dissociation of a specific desired tissue. Common secondary enzymes are: Deoxy-ribonuclease I (DNase I), Elastase, Hyaluronidase and Dispase. During the process of tissue dissociation, parts of the cells are lysed, and the release of monomolecular DNA may cause clumping of cells. To avoid it DNase I is usually added to the dissociation blend. Other enzymes are usually used together with Collagenase for example, Elastase for the isolation of cells found in extensive intercellular fiber tissues, Hyaluronidase for the isolation of functional viable cells from the extracellular matrix and Dispase (characterized by its mild and gently proteolytic action) for the preparation of primary cells and sub-cultivation of cells. As a solution, we have developed several tissue dissociations ready blend products, each designed for specific research and tissue requirements to upgrade the yield of functional and viable isolated cells in more efficient and improved tissue dissociation workflow.