Fluorescent microbial imaging has emerged as an indispensable tool for microbiological research. The ability to identify and describe specific bacteria and other microorganisms has been instrumental in the emerging field of the microbiome. Furthermore, multiplexing combined with high resolution microscopy enables in depth microbial community characterization, in situ.
Here, we examine different tools that we offer for the highly evolving microbiologist toolbox: fluorescent antibiotics, fluorescent metabolic labeling and specific FISH probes for bacteria and fungi. Using these tools can provide data for an extensive set of topics ranging from bacterial colonization dynamics, host physiology and clinical diagnostics to antibiotics mode of action (MOA), antibiotic resistance and toxicity. We will focus on our currently available products and strategizes as well as on future perspectives in this field. We strongly believe that enhancing and diversifying high quality probes for microbiological research will inevitably open new venues for high-standard and complex microbiome related research.