Wilms` tumor diagnosis by Cytoscan HD array
2Sackler Faculty of Medicine, Tel Aviv University, Israel
3Felsenstein Medical Research Center, Tel-Aviv University, Israel
4The Joan and Sanford Weill Pediatric Hematology Oncology and Bone Marrow Transplantation Division, Ruth Rappaport Children's Hospital, Rambam Health Care Campus, Israel
Wilms` tumor is the most common malignant renal tumor in children. The tumor originates in fetal cells, which are involved in the development of the kidney during fetal life, and are not normally be found in the kidney after birth.
Chromosomal changes located on chromosomes 1p and 16q were determined to be statistically significant prognostic factors for classifying the patient into a high-risk group. Patients classified as a high-risk group have a high chance of disease recurrence, and will receive aggressive treatment even with good histology.
In order to improve and refine the classification of Wilms patients into risk groups, the use of Cytoscan HD array (Affymetrix) technology for diagnosis was validated. The CytoScan HD array is a cytogenetics microarray that designed to provide the most comprehensive coverage and highest performance for detecting chromosomal aberrations such as gain, loss and LOH.
Diagnosis by Cytoscan HD array is ֲ accurate, meets international standards and provides comprehensive genomic information directly from the patient sample, based on tens of thousands of genomic probes. This is in contrast to the commonly used method, in which the classification was done comparatively to the patient`s blood and according to few microsatellites.
In addition, diagnosis by Cytoscan HD array provides information on the entire genome, allowing easy adjustment to changes in treatment protocols. ֲ