The Suitability of Bovine AdiposeAdipose-Derived Mesenchymal Stem Cells as a Cell Source for Cultured Meat Applications

Mesenchymal stem cells (MSCs) are known for their plasticity and potential to differentiate into several different cell types; these characteristics have implications for cell therapy, and biotechnological applications, . One of these applications isand in cultured meat production. Stem cellsMSCs used for cultured meat production can be acquired or derived in several ways, from multiple tissues. Adipose-derived MSCs can be isolated. The most common method is taking a cell sample from a live animal . This can be done usingby minimally invasive methods.

In this study, we successfully isolated and characterized mesenchymal stem cells (MSC) derived from freshly adipose tissue 3-month-old calf and investigated the culture conditions required for long-term culturing of these cells. Cells isolated using enzymatic digestion of bovine adipose-tissue biopsy. Samples were shown to grown in vitro for at least 20 passages, verifying their capacity to proliferate. These cells were subjected to immunophenotypic characterization for the presence of CD44, CD29, and CD73, and the absence of CD45, positive and negative MSC markers.

To prove their multipotency, the cells were induced to differentiate into two different cell types, chondrocytes, and adipocytes, which were stained with tissue-specific dyes (Chondrogenic-Alcian Blue, and Adipogenic-Oil-Red O, respectively) to confirm differentiation.

Our results suggest that adipose tissue from bovines can be used as a source of MSC, making adipose tissue-derived cells an interesting option for cultivated meat and biotechnology.