Translation errors in human tisues and multi-target RNA vaccine design

As with other processes in cell, translation can result in more than one outcome for the same RNA. One process that can lead to that is the insertion of the wrong amino acid. An immediate question that is arises from this notion is whether it is a stochastic process that is balanced in a way that minimizes damages to the cell, or is it a deterministic, well-directed process that benefits the cells in a more active way. We looked for sequence related context of amino acids substitutions and explored the conservation of translation errors sites and patterns among different organism and human tissues. Based on that, we aim to design an RNA vaccine that harnesses translation infidelity to instruct cells to create multiple protein sequences from the same mRNA sequence. This technology can be applied to target different variants of a virus that differs from each other by only a few mutations.